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61.
62.
Organic phosphorus (Po) exists in many chemical forms that differ in their susceptibility to hydrolysis and, therefore, bioavailability to plants and microorganisms. Identification and quantification of these forms may significantly contribute to effective agricultural P management. Phosphatases catalyze reactions that release orthophosphate (Pi) from Po compounds. Alkaline phosphatase in tris-HCl buffer (pH 9.0), wheat (Triticum aestivum L.) phytase in potassium acetate buffer (pH 5.0), and nuclease P1 in potassium acetate buffer (pH 5.0) can be used to classify and quantify Po in animal manure. Background error associated with different pH and buffer systems is observed. In this study, we improved the enzymatic hydrolysis approach and tested its applicability for investigating Po in soils, recognizing that soil and manure differ in numerous physicochemical properties. We applied (i) acid phosphatase from potato (Solanum tuberosum L.), (ii) acid phosphatases from both potato and wheat germ, and (iii) both enzymes plus nuclease P1 to identify and quantify simple labile monoester P, phytate (myo-inositol hexakis phosphate)-like P, and DNA-like P, respectively, in a single pH/buffer system (100 mM sodium acetate, pH 5.0). This hydrolysis procedure released Po in sequentially extracted H2O, NaHCO3, and NaOH fractions of swine (Sus scrofa) manure, and of three sandy loam soils. Further refinement of the approach may provide a universal tool for evaluating hydrolyzable Po from a wide range of sources. 相似文献
63.
Phosphorus distribution in dairy manures 总被引:1,自引:0,他引:1
The chemical composition of manure P is a key factor determining its potential bioavailability and susceptibility to runoff. The distribution of P forms in 13 dairy manures was investigated with sequential fractionation coupled with orthophosphate-releasing enzymatic hydrolysis. Among the 13 dairy manures, manure total P varied between 4100 and 18,300 mg kg(-1) dry matter (DM). Water-extractable P was the largest fraction, with inorganic phosphorus (P(i)) accounting for 12 to 44% of manure total P (1400-6800 mg kg(-1)) and organic phosphorus (P(o)) for 2 to 23% (130-1660 mg kg(-1)), respectively. In the NaHCO(3) fraction, P(i) varied between 740 and 4200 mg P kg(-1) DM (4-44% of total manure P), and P(o) varied between 340 and 1550 mg P kg(-1) DM (2-27% of total manure P). In the NaOH fraction, P(i) fluctuated around 200 mg P kg(-1) DM, and P(o) ranged from 130 to 630 mg P kg(-1) DM. Of the enzymatically hydrolyzable P(o) in the three fractions, phytate-like P dominated, measuring 26 to 605 mg kg(-1) DM, whereas monoester P and DNA-like P were relatively low and less variable. Although concentrations of various P forms varied considerably, significant correlations between manure total P and certain P forms were observed. For example, H(2)O-extracted P(i) was correlated with total manure P (R(2) = 0.62), and so was NaOH-extracted P(o) (R(2) = 0.81). Data also show that the amount of P released by a single extraction with sodium acetate (100 mM, pH 5.0) was equivalent to the sum of P in all three fractions (H(2)O-, NaHCO(3)-, and NaOH-extractable P). Thus, a single extraction by sodium acetate buffer could provide an efficient evaluation of plant-available P in animal manure, while the sequential fractionation approach provides more detailed characterization of manure P. 相似文献
64.
65.
Jayson K. Harper Ronald C. Griffin 《Journal of the American Water Resources Association》1988,24(6):1317-1327
ABSTRACT: Texas river authorities are a type of large, regional water district that must be financially self-sufficient. An institutional and historical study of Texas river authorities reveals the broad power of these organizations and their influence in water management. River authorities now control 25 percent of surface water deliveries in Texas. Over two-thirds of authority water was developed by river authorities; nearly one-third was purchased from private or public ventures. While river authority activities have been effective where these services are marketable, the provision of public good services is limited. Increased visibility of these organizations is paralleled by challenges to their traditional autonomy. 相似文献
66.
Ronald C. Griffin Bruce A. McCarl 《Journal of the American Water Resources Association》1989,25(1):175-186
ABSTRACT: Policies to encourage brush management are under consideration as a means to address the water scarcity issue in Texas. Additional water can be generated by treating some of the 100-million-plus acres of brush-infested rangelands in Texas. Evidence of water yield benefits are, however, tentative at this time. Economic investigations based on available data show the potential desirability of brush management but also show benefits to be critically dependent on added water yield, value, and cost-sharing policy. Wildlife, water rights, and environmental issues are also important considerations. The lack of research information on likely impacts makes it difficult to choose among alternative policies for encouraging brush management. More research on this potential opportunity is needed. 相似文献
67.
Charles P. Becker Allender M. Griffin Carol S. Lown 《Journal of the American Water Resources Association》1973,9(5):963-975
ABSTRACT: Water resource and water quality management planning depend, to a large degree, on forecasts of industrial activity and population projections. A flexible economic data base is especially important where planning follows varying formats of geographical and industrial detail. Records of employment and payroll are collected in the administration of Unemployment Insurance (U.I.) programs and are available from State Employment Agencies. These statistics have been collected over a long period of record (thirty-five years). Many years of record are available on punched-cards or magnetic tape and may be arrayed and manipulated by computer. This basic approach has been followed in Virginia. Historical U.I. payroll and employment records for the period 1956 through 1970 were procured on magnetic tape. This data was arrayed by major hydrologic area and by regional planning district. Projections of manufacturing activity were then generated by fitting several exponential equations to annual payroll data in two-digit Standard Industrial Classifications. These exponentials were then extrapolated to provide a range of industrial projections. Other parameters of manufacturing activity were then correlated to the payroll data to generate projections of indexes such as employment, value-added, and gross manufacturing output. U.I. payroll data is now being correlated to parameters in non-manufacturing categories. Projections for industries such as trade and services will link extrapolated payroll data with benchmark correlations of payroll and sales receipts. 相似文献
68.
Jianjun Chen Robert J. Griffin 《Atmospheric environment (Oxford, England : 1994)》2005,39(40):7731-7744
The biogenic species α-pinene, β-pinene, and d-limonene are among the most abundant monoterpenes emitted globally. They are also important precursors to secondary organic aerosol (SOA) formation in the atmosphere. This study involves the development of proposed oxidation mechanisms for these three species. Semi- and non-volatile oxidation products with the potential to lead to SOA formation are predicted explicitly. Simulation code that describes the gas-phase oxidation mechanisms including reactions that lead to ozone (O3) formation is coupled to an equilibrium absorptive partitioning code. The coupled model is used to simulate both gas-phase chemistry and SOA formation associated with oxidation of these three species in chamber experiments involving single as well as multiple oxidants. For the partitioning model, required molecular properties of the oxidation products are taken from the literature or estimated based on structural characteristics. The predicted O3 and SOA concentrations are typically within ±50% of measured values for most of the experiments except for the experiments with low initial hydrocarbon concentrations and the nitrate radical experiments with α-pinene. The developed model will be used to update a gas-phase chemical mechanism and a SOA formation module used in a three-dimensional air quality model. 相似文献
69.
Prenatal diagnosis of cystic hygromata by means of ultrasound examination is possible and highly accurate. Although this is usually associated with Turner's syndrome especially if there is co-existent hydrops fetalis we report three cases in which cystic hygromata were associated with trisomies; two cases of trisomy 21 and one of trisomy 18. 相似文献
70.
DuBose B. Griffin Sally R. Murphy Michael G. Frick Annette C. Broderick John W. Coker Michael S. Coyne Mark G. Dodd Matthew H. Godfrey Brendan J. Godley Lucy A. Hawkes Thomas M. Murphy Kristina L. Williams Matthew J. Witt 《Marine Biology》2013,160(12):3071-3086
From 1998 to 2008, 68 adult female loggerhead sea turtles (Caretta caretta) were instrumented with platform transmitter terminals at nesting beaches in Georgia, North Carolina (NC) and South Carolina (SC) on the East Coast of the United States of America (30°48′N, 81°28′W to 33°51′N, 77°59′W). The majority of post-nesting loggerheads (N = 42, 62 %) migrated to foraging habitats in the Mid-Atlantic Bight during May–October, with a subsequent migration occurring during November–March to foraging habitats south of Cape Hatteras, NC. Nine (13 %) loggerheads initially foraged in the near-shore, coastal areas of the South Atlantic Bight, but moved to offshore habitats—closer to the Gulf Stream—during November–March, while fourteen (21 %) loggerheads remained in foraging areas along the mid-continental shelf off of the eastern coast of Florida and/or continued southward to Florida Bay and the Bahamas. The present study delineates important, post-nesting foraging habitats and migration corridors where loggerheads may interact with commercial fisheries—providing managers opportunities to develop and implement optimally effective conservation actions for the recovery of this threatened species. 相似文献